These proteins include those with direct microtubule binding activity, such as the KL1, Mis12, Ndc80 complex (Ndc80C) (together forming the KMN network), CENP-E, and the Ska1 complex, all of which are transient factors that modulate this interaction or monitor attachment status.
The spindle checkpoint monitors the attachment of homologous pairs of sister chromatids to opposite poles of this spindle at Meiosis I (Shonn et al. 2000).
The spindle assembly checkpoint monitors the attachment of the kinetochores to the microtubules; and two proteins, called Bub1 and BubR1, play an essential role in this process.
Although this checkpoint formally monitors chromosome attachment to the spindle, there are at least two components addressed here that potentially impact the SAC, centrosome anchorage and nuclear occupancy.
Complete APC/CCdc20 activation also requires inactivation of the spindle assembly checkpoint (SAC), which monitors proper attachment of sister chromatids to the spindle [ 17, 18].
DOI: http://dx.doi.org/10.7554/eLife.05269.002 Bub1 and BubR1 are paralogous proteins involved in the spindle assembly checkpoint (SAC), a safety device that monitors the attachment of kinetochores to spindle microtubules and halts mitotic progression until completion of chromosome bi-orientation on the mitotic spindle (Lara-Gonzalez et al., 2012; Foley and Kapoor, 2013).
The centromere is a specialised chromosomal structure that regulates faithful chromosome segregation during cell division, as it dictates the site of assembly of the kinetochore, a critical structure that mediates binding of chromosomes to the spindle, monitors bipolar attachment and pulls chromosomes to the poles during anaphase.
We considered whether to add a cerebral regional oxygenation (rSO2) monitoring, but attachment of rSO2 sensors to his forehead was difficult in the prone position.
The suitability of the quartz crystal microbalance (QCM) technique for monitoring the attachment and spreading of mammalian cells has recently been established.
An engineered Shewanella oneidensis MR-1 (ATCC700550) producing eGFP was used as electro-active model organism to demonstrate the practical application of the flow cell as BES to monitor cell attachment simultaneously with EIS and CLSM.
