We can monitor assembly of TBP, Brf2, Bdp1, and SNAPc on a U6 promoter by EMSAs [25], and so we checked whether addition of recombinant human Maf1 inhibited formation of either the four-factor complex or various subcomplexes on the U6 promoter.
By utilizing FRET signals from fluorescently labeled P/T DNA and PCNA to monitor protein-DNA interactions, we were able to observe both activities of RFC in real time and directly monitor assembly of the DNA pol holoenzyme.
We used mass spectrometry (MS) to monitor assemblies formed between eukaryotic Hsp90/Hsp70/Hsp40, Hop, p23, and a client protein, a fragment of the glucocorticoid receptor (GR).
For example, previous work suggested the existence of a checkpoint that monitors septin assembly and induces a Swe1-dependent G2/M delay in response to defects in septin assembly [63], [64].
Occupational therapist and athletic trainer Deana Zornow, left, monitors an assembly worker at Tesla's Fremont, CA, plant, providing posture and exercise tips to reduce repetitive stress and strain injuries.
Our results suggest a novel cell cycle surveillance mechanism that monitors nucleosome assembly without involving the DNA repair pathways and exerts its effect via suppression of CDC25 phosphatase String expression.
We use this framework to study the spindle checkpoint, the process that monitors the assembly of the mitotic spindle and blocks cell cycle progression at metaphase until all chromosomes have made bipolar spindle attachments.
Thickness of ZIF-8 shell can be simply tuned by monitoring the assembly cycles.
UV vis, fluorescence spectroscopy, XRD, SEM and AFM have been adopted to monitor the assembly process.
Piezoelectric quartz crystal impedance (PQCI) technique was employed to monitor the assembly of DNA in real time.
