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After 20 minutes of incubation at room temperature, naphthol AS-MX phosphate and fast red TR salt were added, followed by incubation at 37°C for 1 hour with close monitoring under the microscope after the first half hour to monitor the development of a bright red staining for osteoclastic activity.
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When the inner vesicle or the junction between the micropipette and the nanotube is subjected to the translation movement along the LNT axis, the length of the tube is changed (increasing or decreasing its value in a controlled way, which can be monitored under the microscope).
The growth characteristics and the differentiation were monitored under the microscope (Nikon, TE2000U) and were characterized.
Because the nematode behaviors can be easily monitored under the microscope, another sensitive endpoint, movement or locomotion behavior, was also monitored using a computer tracking system after metal exposure in nematodes [23] [26].
Cell disruption was monitored under the microscope.
The resultant color development of DAB was monitored under the microscope.
DOI: http://dx.doi.org/10.7554/eLife.06197.009 We then used a temperature-controlled microfluidic device that enabled us to categorize cells depending on their pre-stress age-associated protein deposit-status, and monitor them under the microscope prior to, during and after undergoing acute proteotoxic stress conditions.
However, QDAβ is a useful nanoprobe, if used as a small fraction of the unmodified Abeta, for monitoring the aggregation under the microscope.
Selected CRC cells, including HCT116, Caco-2, LoVo, and SW480, were monitored under the fluorescence microscope after virus infection to see the functional effect of HOXB13.
The progress of autolysis was also monitored by observing cells under the microscope (magnified 1000 times) and comparing them to cells from the original culture.
By carefully monitoring under a light microscope, the distance migrated by the cells over the indicated time periods was measured and quantified.
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