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Bianco, L. & Bessant, C. Free computational resources for designing selected reaction monitoring transitions.
The use of two selected reaction monitoring transitions for each compound allowed simultaneous quantification and identification in one run.
Quantification was achieved by monitoring transitions of m/z 422.1 → 285.4 for lovastatin and 425.4 → 285.4 for lovastatin-d3 in multiple reaction monitoring, using turbo ion source in positive polarity.
The most abundant transition was found to be fragmentation of the formate adduct to the molecular ion giving rise to the following selective reaction monitoring transitions of 287→241 and 290→244 for thymidine and D3-TdR, respectively.
Multiple reaction monitoring transitions of individual lipid species were listed in Table 1 (Table 1).
The characteristic multiple reaction monitoring transitions of MMMTAV were 155/107, 155/121, and 155/137.
The monitoring transitions for levosulpiride and the IS were 342.0→112.0 and 349.1→206.1, respectively.
The multiple reaction monitoring transitions monitored were 366.04 > 144.04 m/z and 383.02 > 161.06 m/z for α-AASA and N-aminoadipic acid, respectively.
The characteristic diagnostic ions for multiple reaction monitoring transitions are [PO2]- at m/ z 63 and [PO3]- at m/ z 79.
All analytes and their respective labeled standards were identified using their specific retention time and two multiple reaction monitoring transitions (Table S2, Supporting Information).
The multiple reaction monitoring transitions monitored were 113.70 > 43.90 m/z and 116.70 > 46.90 m/z for creatinine and creatinine-d3, respectively.
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