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A FAM-labeled, random sequence pre-miR was used for monitoring transfection efficiency by flow cytometry.
Plasmid Renilla luciferase (PRLSV40) was used as internal control for monitoring transfection efficiency.
pCMVβ-galactosidase plasmid (Clontech, Palo Alto, CA, USA) was used for monitoring transfection efficiency.
For monitoring transfection efficiency pSV40-renillaLuc was cotransfected.
EGFP expression served as an internal control for monitoring transfection efficiency.
The EGFP expression plasmid served as an internal control for monitoring transfection efficiency.
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pRL-Renilla reporter vector (Promega), which serves to monitor transfection efficiency, was co-transfected with the pGL2 plasmid.
As transfection control, pSV β-galactosidase vector (1.0 μg) was co-transfected into the cells to monitor transfection efficiency.
To monitor transfection efficiency, 0.1 µg pRL-SV40 was transfected as an internal luciferase control.
Each sample was co-transfected with 0.05 μg pRL-CMV plasmid expressing Renilla luciferase to monitor transfection efficiency (Promega Corporation).
Purified human CD4+ lymphocytes from normal and HIV-1 positive donors were transfected with plasmids encoding for procaspase 8, or Casp8p41, or GFP to monitor transfection efficiency.
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