Sentence examples for monitoring transduction efficiency from inspiring English sources
The presence of an IRES sequence followed by the green fluorescent protein (GFP) gene in the MIGR1 vector allows a co-expression of c-Rel siRNA duplex and the GFP, the latter being used for monitoring transduction efficiency.
A lentiviral vector system containing a GFP reporter gene to monitor transduction efficiency was used, and altered NDRG1 expression level was verified using western blot analysis (Fig. 1A).
Lentiviruses expressing GFP (Lenti-GFP) were used to monitor transduction efficiency, and those containing no shRNA or non-target shRNA (Lenti-sh) were included as controls.
Genetically engineered trackable markers and imaging reporters enable noninvasive monitoring of transduction efficiency and pharmacokinetics of anticancer virotherapeutics.
Each virus also encodes the green fluorescent protein (GFP) for the microscopic monitoring of transduction efficiency and ∼95% of the Schwann cells were transduced, as visualized by the presence of the green fluorescence (data not shown and Figure 1).
HSCs were monitored for transduction efficiency, surface marker expression and cellular function.
The transduction efficiency monitored by measurements of green fluorescence encoded by the reporter gene GFP reached 85% (Fig. 1a).
The process was continuously monitored to determine kinetics of expansion, transduction efficiency and phenotype of the engineered cells in comparison with small-scale transductions run in parallel.
It also expresses the Zoanthus Green Fluorescent protein (ZsGreen) gene via an IRES sequence, which can be used to monitor and normalize transduction efficiencies.
We also provided to Addgene different control vectors to monitor transfection and transduction efficiencies and test for possible toxic effects of the viruses, including some insert-less vectors, or vectors encoding cDNAs or hairpins against GFP or luciferase (see Table S1 for details).
The transduction efficiency and stability of the transfectants were monitored by GFP FACS analysis.
