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The newly developed biophysical assay provides a tool for monitoring the toxicity levels of drug treatment on animal development.
Here we describe the construction of whole cell genetically modified bioluminescent biosensors and their immobilisation for use in monitoring the toxicity of a complex industrial wastewater containing phenolic materials.
The system is also useful for monitoring the toxicity and side effects of drugs at the organismal level.
Recently, this platform has proved very informative in monitoring the toxicity of compounds [ 29], biomaterials [ 30], inhibitors [ 31] and the cell differentiation process [ 32, 33].
Toxicogenomics, including in its broader scope transcriptomics and proteomics, is a promising tool not only for monitoring the toxicity of substances using human cell line assays but also for investigating and documenting the modes of action of unknown chemicals [ 8- 12].
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We seek to expound on how these nanomaterials communicate in the complex environment to reach the target site, and how to design the effective TDDS for complex environments and simultaneously monitor the toxicity on the basis of designing such delivery complexes.
As stated recently: "Real-time imaging of cell death would be a coveted application with which to assess the efficacy of cytotoxic drugs and, potentially, to monitor the toxicity of these drugs in normal tissues" [14].
To monitor the toxicity of the treatment, the body weights of the mice were weighed.
Assays using C. elegans to observe the lethality and sublethal endpoints, growth, reproduction, lifespan, locomotion behavior, stress response, and oxidative damage have been well developed to monitor the toxicity of heavy metals including Cr VI) [ 8].
Decreased cholinesterase activity is used to monitor the toxicity of some pesticides such as organophosphate and carbamates that are associated with the development of liver diseases, peripheral neuropathy, neuropsychiatric abnormalites and extra pyramidal disorders [ 21- 27].
GC2 (lac::luxCDABE), a constitutively bioluminescent strain, was used to monitor the general toxicity of samples through a decrease in its bioluminescence, while specific toxicity was detected through the use of strains such as DPD2540 (fabA::luxCDABE), TV1061 (grpE::luxCDABE), DPD2794 (recA::luxCDABE), and DPD2511 (katG::luxCDABE).
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Justyna Jupowicz-Kozak
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