In order to monitor the specificity of CKII phosphorylation, 4,5,6,7- tetrabromobenzotriazole (TBB), a specific inhibitor of CKII [34], was utilized.
Thermal conditions were: 94°C for 10 s as the first denaturing step, followed by 94°C for 5 s, 55°C to 60°C for10 s, and 72°C for 15 s, for 40 cycles, followed by a gradual increase in temperature from 55°C to 96°C during the dissociation stage (to monitor the specificity of each primer set).
Mouse melanoma B16F10 cells are HPV-negative and were used to monitor the specificity of Cidofovir action.
At the end, samples were subjected to a slow temperature-ramping dissociation stage of 15 seconds at 95°C, 15 seconds at 60°C, 15 seconds at 95°C to monitor the specificity of amplification.
In a first series of experiments, we explored the contribute of spatial factors in time processing (past vs. future) using verbs and modifying their tense to monitor the specificity of semantic motor activations for spatial representation of past and future.
We first monitored the specificity of the knockdown effects.
To monitor the specificity of the amplification, melting curve analysis was performed after each PCR.
To monitor the specificity, final PCR products were analyzed by melting curves.
To monitor the specificity of reaction, IgG was used as an isotype control.
