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To test the chaperone function of MAC with HGD, we used two different aggregation conditions: (a) as in ref (30), using a concentration jump from high denaturant to low denaturant concentration (from 6 to 0.5 M GdnHCl) and monitoring the resulting scattering over a period of time, and (b) heating HGD to 68 °C and monitoring the scattering for a similar time period.
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Directly above the sample, an optical system with a light-scattering detector continuously monitors the scattered intensity signal (S) of the sample as it is subjected to prescribed temperature alterations.
The extent of aggregation was measured by monitoring the light scattering at 360 nm using Shimadzu UV 160 spectrophotometer equipped with a temperature regulated cell holder.
Rhodanese aggregation was followed by monitoring the light scattering at 320 nm for 5 min. IgY was purified using the Chicken IgY Purification kit (Thermo Fisher Scientific) and suspended in 0.1 M Tris HCl, pH 8, at a final concentration of 50 mg/ml.
Van der Meer, in response to this problem, devised a mechanism that would monitor the particle scattering at a particular point on the ring and would trigger a device on the opposite side of the ring to modify the electric fields in such a way as to keep the particles on course.
Sonkaria et al. monitored the light scattering intensity and revealed that 1 mmol/L of Ca2+ could maximize the polymerization efficiency of MSR-1 MamK (Sonkaria et al., 2012).
One day, I went with a small American force of two tanks and a couple of Humvees as they swept one of the highways on the outskirts of Baquba just after dawn, looking for potential IEDs and monitoring the Iraqi army checkpoints scattered on that road.
As of Thursday, the militant group controls more than half of Syria, according to the Britain-based Syrian Observatory for Human Rights, an opposition group monitoring the war through activists scattered around the country.
To achieve these scientific goals, GIADA is composed of different modules: an optical detection system, to monitor the light scattered by single particles entering the instrument, an impact sensor, to detect the momentum carried by the particles, and five micro-balances, to measure the dust flux from different directions.
These particles also displayed spontaneous and complete redispersion upon cooling as monitored by the scattering intensity.
Because of the absorption of light by proteins at lower wavelengths, nanosheet self-assembly by SbpA was monitored via the scattering of light at 340 nm.
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