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On the contrary, here we aimed to perform our experiments respecting the physiological stoichiometry of protein/mRNA interactions by monitoring the localization of native RNAs and protein.
Intravenously injected 4T1 cells expressing a luciferase reporter allow monitoring the localization of cells by life imaging.
Similarly, monitoring the localization of Dvl-GFP by immunofluorescence, we also found increased Dvl nuclear residency in Wnt5a-expressing cells.
Determining the effect of specific mutations often requires monitoring the localization of the protein in cultured cells and the level of cellular iron accumulation.
Using this strain, we investigated whether eS26 is required for nuclear export of pre-40S subunits and/or cytoplasmic 20S pre-rRNA processing by monitoring the localization of uS5-GFP and Cy3-ITS1.
At an ultrastructural level, BMECs co-cultured with differentiating NPCs possessed a higher percentage of smooth and continuous tight junctions as determined by monitoring the localization of proteins such as claudin-5, occludin, and ZO-1.
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Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells.
One of the main applications of the transient gene assay tools is to monitor the localization of proteins to sub-cellular compartments in living cells.
While we did not monitor the localization of ICP0 within our previous study, others have shown that ICP0 RING finger mutants localize predominantly within the nucleus [22], [63].
To determine the subcellular localization of AtGILP, we constructed a fusion of the green fluorescent protein (GFP) gene and AtGILP under the control of the CaMV 35S promoter and monitored the localization of GFP-AtGILP in Arabidopsis mesophyll protoplasts.
We carefully monitored the localization of ICP0 as recent studies have begun to address the biological relevance of its localization during the course of an HSV-1 infection [19], [35], [37], [41], [42].
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identified the localization
controls the localization
monitored the localization
monitoring the situation
monitoring the impact
monitoring the site
monitoring the budget
monitoring the industry
monitoring the ship
monitoring the sugar
monitoring the state
monitoring the company
monitoring the phone
monitoring the conversation
monitoring the country
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