Sentence examples for monitoring the internalization from inspiring English sources
We followed integrin α5β1 endocytosis by monitoring the internalization of the anti-α5β1 antibody P8D4 (Figure 8).
Here, we reported a useful experimental tool named as T-REx-c-MIR for monitoring the internalization induced by surface ubiquitination.
This was accomplished by attaching an octa-arginine tail known to facilitate membrane translocation and a fluorescein probe for monitoring the internalization.
To monitor the internalization and intracellular localization of the dual labeled TP2/pDNA complex (the best system in terms of transfection efficiency) in HeLa cells, confocal microscopy was used.
Using a GLP-1 conjugated to tetramethyl rhodamine to monitor the internalization of the receptor, Kuna et al. [ 37] observed that after internalization, GLP-1R/ligand complex could be co-localized with adenylate cyclase in the endosome.
We monitored the internalization of the fluorescent complexes 47· 48 and CB[7]· 48 into murine lymphocytic leukemia cells (L1210) and their derived cell line L1210FR, which overexpresses biotin receptors, by confocal microscopy and flow cytometry.
We next monitored the internalization of surface-biotinylated EGFR in response to EGF followed by quantitative analysis by densitometry, as biotinylation is widely used to selectively mark surface-exposed proteins including EGFR without reported alterations of their trafficking or function (Choi et al, 2004; Elia, 2008).
This pathway was further investigated by monitoring the cellular internalization of fluorescent LDL-[BODIPY-Cho].
To monitor further the internalization and intracellular transport of TAT-SA and SA, transduced HeLa cells were labeled with an antibody against SA and nanogold-immunolabeling electron microscopy with a silver enhancement technique was used.
The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo.
To monitor the antibody-induced internalization of cell surface MICL, freshly isolated neutrophils were incubated with 50C1 antibody (1 µg/ml) or IgG2a isotype antibodies for the indicated times at 37°C and then washed, and the resuspended cell pellets were incubated for a further 30 min on ice with FITC-labeled goat antimouse Fcγ-specific IgG (diluted 1 100 in HBSS/BSA solution).
