Sentence examples for monitoring the fluorescent from inspiring English sources

This finding is based on monitoring the fluorescent fraction of secreted AG Es which has the typical peak at 440 nm and a broad spectrum, reflecting the heterogeneity of the fraction (see insert of Figure 1A).

We took advantage of this property to observe the rise of intracellular Ca2+ concentration provoked by the influx of ions through the peptide-permeabilized plasma membrane by monitoring the fluorescent GFP-protein binding to the plasma membrane.

NO released into the culture medium was measured by monitoring the fluorescent compounds created by 3-diaminonaphthalene exposed to NO2/NO3.

The threshold cycle (CT) was determined by monitoring the fluorescent signal for each cycle, and the amounts of mRNA in the experimental groups were determined relative to those of the control group.

After amplification, melting curve analysis was performed by heating the sample to 95°C for 15 s, then cooling it to 70°C for 1 min, followed by a linear temperature increase to 95°C at a rate of 0.5°C/s, while continuously monitoring the fluorescent signal.

To determine the specificity of the primers used for PvTIFY10C and PvEF1, after completion of the amplification reaction, a melt curve was generated by increasing the temperature in small increments and monitoring the fluorescent signal at each step following the protocol: 1 min at 95°C, 1 min at 55°C, 10 sec at 55°C (80 cycles, increasing by 0.5°C each cycle).

The effect of exposure to 12 Co-PCB congeners and TCDD on transcription levels of pb-1, pb-2 and pb-3 was investigated using real-time quantitative RT-PCR to monitor the fluorescent intensity of SYBR Green.

Additionally, we monitored the fluorescent pattern of BBB-NPs treated cells over a time-course of 7 d.

We monitored the fluorescent ROS reporter 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) by flow cytometry.

RT Real-TimeTM SYBR Green/fluoresceincludedaster Min (included in the kit) was used to monitor the fluorescent signal during each cycle of the PCR reaction.

To evaluate this protocol, we monitored the fluorescent signal of hChR2(H134R -mCherry's reporter protein in 3 sister cultures over tH134R -mCherrynfection using an LSM510 confocal microscope (Carl Zeiss AG, Oberkochen, Germany).