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The synthesized compound proved to be a useful substrate for monitoring the enzyme activity of sphingomyelinase by detecting the liberated thiol group with a thiol-sensitive reagent.
MEP synthase activity was evaluated at 22°C by spectrophotometrically monitoring the enzyme catalyzed oxidation of NADPH using an assay derived from that described by Takahashi et al [4].
The storage stability of the α-amylase in the toothpaste at room temperature was determined by monitoring the enzyme activity for ten months.
Complex II activity was measured in the same reaction mixture by adding 50 μM DB and monitoring the enzyme kinetic for 3 min.
The NIR 'window' from 680 to 980 nm is also key to monitoring the enzyme in mitochondria, intact cells and whole organisms [24,25] and so to linking enzymatic events with global oxygen metabolism in vivo [26].
The biodistribution and PET imaging studies on animals revealed that F-labeled GCase (40) is a powerful tool for monitoring the enzyme distribution and tissue half-life in vivo by PET with an immediate clinical application to Gaucher disease.
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We conclude, with some caveats, that the 830 nm band is indeed largely due to cupric CuA and that this monitors the enzyme turnover, as previously suggested.
We added varying amounts of non-radioactive pEndos to PP2A-B55, and monitored the enzyme's ability to dephosphorylate radioactive pCDKS added at the same time.
Reference DNA sets (included unmethylated DNA and methylated DNA) (QIAGEN #59695, QIAGEN, Inc., Valencia, CA, USA) were used to monitor the enzyme digestion efficiency.
We thus performed steady-state assays to monitor the enzyme kinetics of YcgC via measuring the production of the downshifted RutR band, and estimated the Km and Vmax of YcgC to be 2.13 ± 0.65 μM and 0.29 ± 0.07 μM/min/μM, respectively.
The effect of the pH, temperature, and NaCl concentration on the enriched amylase enzyme activity was evaluated by sequential univariate analysis of the pH, temperature, and NaCl concentration, respectively, and monitoring the relative enzyme activity (the highest activity was referred to as 100%) as the selected parameter.
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