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Importantly, the probe could be applied in monitoring the endogenous production of H2S in raw264.7 macrophages.
We here designed a NIR fluorescent probe for monitoring the endogenous production of H2S in living cells.
Monitoring the endogenous levels of Asf1a and Asf1b revealed that both are normally down-regulated in mitosis (Figure 8B).
Senescence was assessed by monitoring the endogenous B-galactosidase (B-gal) activity at pH 6 (Fig. 1B).
Because low resistance seals (<20 MΩ) do not influence the cell membrane and because the normal intracellular milieu is maintained, this approach is the least invasive method for monitoring the endogenous electrical activity of single cells.
Similar(54)
Imaging procedures to monitor the endogenous autofluorescence signal from NAD(P H during activation of the NADPH oxidase have been developed (Liang and Petty 1992).
We also monitored the endogenous VAPB and PTPIP51 interaction in cells using an in vivo, in situ proximity ligation assay (31).
Finally, the difficulty of monitoring the concentrations of endogenous ligands in the tissue of interest has complicated the interpretation of the studies of GPCR activation in vivo.
We next evaluated autophagosome formation by directly measuring the cellular distribution of endogenous LC3B and by monitoring the autophagic flux, as determined by comparing the accumulation of autophagosomes after the inhibition of lysosomal proteolysis with NH4Cl/leupeptin (NL) relative to the steady-state levels of autophagosomes (22).
To monitor the kinetic of endogenous PARP2 recruitment following DNA damage, laser micro-irradiation was performed to generate local DNA damage in U2OS cells.
Immunohistochemistry was also conducted to monitor the distribution of endogenous TR2 and PML NBs in a gain- or loss-of-HDAC3-expression system (Fig. 2C).
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