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Amplification of a single PCR product was confirmed by monitoring the dissociation curve.
The specificity of PCR amplification was examined by monitoring the dissociation curves during qPCR reactions.
This format uses a saturating concentration of ligand for binding, followed by monitoring the dissociation rate for an extended period of time (~2 3 h).
The normalised plot was generated by monitoring the dissociation of the fluorescent dye from double-stranded DNA as the temperature increased.
Although some authors have proposed a consistent, positive Southern analysis as an alternative methodology for monitoring the dissociation of chimeras, our data show that it does not provide enough proof of uniform transformation.
Similar(55)
A protein protein Förster resonance energy transfer (FRET) system, employing probes at multiple positions, was designed to specifically monitor the dissociation of the H2A H2B dimer from the nucleosome core particle (NCP).
The biochip is designed to function as a platform for a competitive label-free immunoassay and two real-time transducers – surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) – are used to monitor the dissociation of on-line immobilised monoclonal antibodies produced against TNT.
This method can be extended to monitor the dissociation of chimeras and the recovery of uniformly-transformed plants.
This result reinforces the view that this technique is not sufficient to discriminate chimerical from uniformly-transformed plants or to monitor the dissociation of chimeras.
In this work, however, real-time PCR was applied successfully to monitor the dissociation of chimeras in tobacco plants and apricot callus.
We next used LDI/MALDI to monitor the dissociation of host guest complexes using ADA, a strong binding competitor for CB[7].
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monitoring the disengagement
monitoring the split
monitoring the cleavage
monitoring the breakdown
monitoring the withdrawal
controls the dissociation
monitoring the situation
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monitoring the industry
monitoring the sugar
monitoring the ship
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