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The etheno protons also provide markers for monitoring the conformational change from the anti to the syn conformations.
The dynamic nature of Sdo1p on the 60S subunit, together with its strategic binding position, suggests a surveillance role of Sdo1p in monitoring the conformational maturation of the ribosomal P-site.
By monitoring the conformational evolution of wild-type protein and variants thereof, either in response or absence of external perturbation, valuable insights into intermediate structure and fibrillogenesis mechanisms are gained.
Tokyo, Japan) equipped with a peltier cell holder and temperature controller PFD425 L/15 was used for monitoring the conformational changes in the protein on alkaloid binding.
Furthermore, as seen from Fig. 5, N-site s7 is in less than 10 Å proximity to the most disordered region of the linker suggesting that this site might be critical for the quality control system in monitoring the conformational transitions in the linker region during the folding process.
The changes in cleavage patterns reveal a series of concerted structural transitions, and illustrate the potential of this method for monitoring the conformational dynamics of complex macromolecular reactions.
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The possibility to monitor the conformational change by means of electrical measurements is identified as the key point for a better knowledge of charge transport in biological materials and for exploiting new frontiers of application of sensing proteins.
To monitor the conformational changes during the simulations, the root mean squared deviation (RMSD) between the initial coordinates of the backbone atoms of the proteins and the coordinates along the MD simulations was computed for every snapshot.
In the present study we have monitored the conformational changes in β′ as the denatured protein is refolded in the presence and absence of ω using tryptophan fluorescence emission of β′ as well as acrylamide quenching of Trp fluorescence.
Together with published data, our results suggest that Sdo1p is an essential surveillance factor for the 60S maturation, which monitors the conformational status of the ribosomal P-site, including surrounding uL16, H69 and H38, and couples, through Efl1p, the maturation of the ribosomal P-site to the release of Tif6p.
In this work, we investigated the structural significance of hTERT Q169 directly by using limited proteolysis to monitor the conformational flexibility of mutant proteins.
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