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The suitability of the quartz crystal microbalance (QCM) technique for monitoring the attachment and spreading of mammalian cells has recently been established.
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We monitored the attachment of the beads as the response of a quadrant diode to bead movement (see Methods).
ECIS allows researchers to monitor the attachment and spreading of mammalian cells in temporal resolution.
The spindle assembly checkpoint monitors the attachment of the kinetochores to the microtubules; and two proteins, called Bub1 and BubR1, play an essential role in this process.
By optical imaging, we monitored the attachment of hdpPSCs on the E-spun fibers in the initial 12 h of culture.
To solve this problem, we developed a continuous fluorescence assay that can monitor the attachment of a coumarin-containing FPP analogue (4) to a GFP variant equipped with a C-terminal CVIA sequence that makes it a substrate for PFTase.
DOI: http://dx.doi.org/10.7554/eLife.05269.002 Bub1 and BubR1 are paralogous proteins involved in the spindle assembly checkpoint (SAC), a safety device that monitors the attachment of kinetochores to spindle microtubules and halts mitotic progression until completion of chromosome bi-orientation on the mitotic spindle (Lara-Gonzalez et al., 2012; Foley and Kapoor, 2013).
The efficient apoptotic elimination of MI spermatocytes with a univalent sex chromosome that fails to achieve bipolar attachment to the spindle (as in X Sxr aO males [ 2, 10, 11]) is widely assumed to be triggered by a spindle assembly checkpoint (SAC) monitoring the bipolar attachment of bivalents to the spindle [ 1].
Results showed this sensor could monitor the cell attachment process in real time and response signals were related to the initial cell seeding densities.
The spindle assembly checkpoint (SAC), or mitotic checkpoint, monitors the microtubule attachment to kinetochores, ensuring the correct segregation of chromosomes [1], [2].
The spindle checkpoint ensures the accurate segregation of chromosomes by monitoring the status of kinetochore attachment to microtubules.
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