We are also developing a single molecule assay to monitor telomerase activity in real time and study the interactions between shelterin, DNA structure and telomerase activity in model telomeric DNA.
These findings suggest that response of malignant glioma cells to TMZ can be monitored by reduction in telomerase activity.
Telomerase activity was monitored in HEp-2 and EL1 monocytes cell lines.
The telomerase activity could be monitored in situ by the fluorescence signal without the need for obtaining cell extracts.
The proposed strategy could be employed for in situ monitoring of the change of intracellular telomerase activity in response to drugs to detect drug efficacy.
Sawant SG, Gregoire V, Dhar S, Umbricht CB, Cvilic S, Sukumar S et al. Telomerase activity as a measure for monitoring radiocurability of tumor cells.
To assess whether telomere shortening in the tpz1-K75A mutant is caused by impaired telomerase activity, heterozygous tpz1 K75A /+ diploids were sporulated, and telomere length was monitored in the tpz1-K75A overpring over time.
Cell proliferation, telomerase activity, telomere length and the expression of multidrug resistance genes were serially monitored as the PDL increased.
Therefore, determination of telomerase activity during or after treatment with chemotherapy or radiation may be a novel measure for monitoring chemo- or radioresponse of tumour cells.
Since telomerase activity is found in the majority of nasopharyngeal cancers and a subset of metaplasia, this enzyme may be served as a reference to monitoring the status of abnormal nasopharyngeal tissues.
