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Due to the high inherent sensitivity of these parameters to changes in tissue architecture (i.e., macromolecular, cellular, tissue, and organ structure) and in its physiologic state, their potential applications include monitoring structural changes in development, aging, and disease.
Measurements of inter- and intramolecular distances are important for monitoring structural changes and understanding protein interaction networks.
In addition, UV vis spectroscopy, CD technique and fluorescence spectroscopy were also used for monitoring structural changes.
Mice deficient in interleukin 4 (IL-4) or in intestinal trefoil factor/trefoil family factor 3 (ITF/TFF3) were infected with T. spiralis and the resultant changes in the intestinal mucosa followed by quantifying numbers of mucosal mast cells, goblet cells, Paneth cells and by monitoring structural changes in villus length and crypt depth.
There were many examples of how HDX MS can be used to study protein/protein interactions, ranging in application from analysis of identifying contact surfaces to monitoring structural changes in one or more members of the complex upon binding.
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C chemical shifts are very sensitive to protein secondary structure and can, therefore, be utilized to monitor structural changes throughout film production.
FTIR, TGA and SEM analysis was applied to monitor structural changes and phase formation processes during thermal treatment.
This paper presents recent developments of the transient plane source techniques, and a discussion about their ability to monitor structural changes.
As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification.
The behavior of silicate glasses under ultrahigh pressures above 100 GPa has recently been explored by acoustic wave velocity measurements using in situ high-pressure Brillouin scattering spectroscopy as a way to monitor structural changes in silicate melts under ultrahigh-pressure conditions (Murakami and Bass 2010, 2011).
The accurate prediction of conductive properties as a function of the physical characteristics of the composite material, will aid in the design of composites that can, for instance actively monitor structural changes due to compression, stretching, or even damage through their effect on the conductivity.
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