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In this study, we describe a simple, highly sensitive assay for monitoring both the secretory pathway and ER stress in living mammalian cells based on expression of the naturally secreted Gluc [18] and monitoring release of luciferase activity in real-time.
Further confirmation of the above results was obtained by culturing peritoneal macrophages from Wt and PrPKO mice overnight, loading them with 59FeCl3-citrate complex after stimulation with LPS [18], and monitoring release of 59Fe into the medium in a γ-counter.
We tested the effect of high ATP or BzATP concentrations in MSC-1 and MSC-2 lines by monitoring release of the cytoplasmic marker lactate dehydrogenase (LDH).
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Monitoring releases from industry to air, water, and land and making this information public is an important strategy for controlling environmental exposures to carcinogens and other toxic chemicals (UN 2013).
These models must also incorporate changes in release due to the dissolution media and methods employed to monitor release.
Moreover, the designed biosensor was able to monitor release of IP3 upon induction by different inducers like Bradykinin and ATP.
Earlier in the day, an OSCE source told the BBC that a negotiating team had been sent to Sloviansk to try to secure the monitors' release.
Here we show that focused ultrasound, used at pressures similar to those applied during diagnostic ultrasound scanning, can be utilised to both trigger and monitor release of payload from liposomes.
These were observed daily to monitor release of ascospores, usually signalled by white spore shadows on the agar surface and/or on the lid.
We also tested MglB-stimulated GTP hydronysis on MglA using the [γ-P]GTP charcoal assay, which monitors release of inorganic Pi.
We monitored release of LDH into the culture medium, which results from the disruption of cell membranes and release of LDH with other cytoplasmic components.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com