Sentence examples for monitoring product accumulation from inspiring English sources
By monitoring product accumulation during rapid amplification, quantitative polymerase chain reaction in a closed-tube system is possible in 15 to 30 minutes.
Product accumulation was monitored by SYBR Green (Kapa Biosystems) fluorescence with Eppendorf Mastercycler ep realplex.
Afterwards, the mixture was diluted with 30 µL H2O and real-time qPCR product accumulation was monitored in a Light-Cycler480 (Roche-Diagnostics) using 1 µL of total cDNA, 312.5 nM forward, 312.5 nM reverse primer and SYBR-Green I Mastermix (Roche-Diagnostics, Mannheim, Germany) according to manufacturer's instructions.
Product accumulation was monitored with a Mastercycler ep Realplex (Eppendorf, Wesseling-Berzdorf, Germany) detection system.
This limitation is fully addressed using real-time qPCR methods [ 54], whereby PCR product accumulation is monitored at each PCR cycle by means of fluorescent detection using molecular beacon chemistry.
Thus, to allow significant product accumulation for accurate measurement, the activity was monitored in the reverse direction, UDP-Gal p from UDP-Gal f.
It means that the product accumulation would be independent of incubation time.
This suggested that no irreversible process or toxic product accumulation occurred when trans-translation was inactivated.
Advanced glycation end product accumulation was measured with AF reader.
–Confirm and monitor product development timelines.
In case of reversible reactions, end-product accumulation can change the direction of the reaction.
