The Gluc secretion assay provides a highly sensitive, facile way to monitor processing of proteins through the secretory pathway.
We have developed a highly sensitive assay to monitor processing of proteins through the secretory pathway and endoplasmic reticulum (ER) stress in real-time based on the naturally secreted Gaussia luciferase (Gluc).
To evaluate whether the death induced by the different ER stressors was relying on caspase activation, we monitored processing of caspase-9 and -3 as well as cleavage of the caspase-3 substrate PARP by immunoblotting cell lysates obtained after stimulating the cells with 0.5 μg/ml of brefeldin A, 2.5 μM of thapsigargin and 2.5 μg/ml of tunicamycin over a period of 24 h.
Monitoring, processing and integration of sensory inputs by the cerebellar circuitry is important for learning and adaptation in order to anticipate sensory events.
In addition, it might also indicate a sort of P3b reflecting monitoring processing and stimulus evaluation.
set-up to in situ real-time monitoring of processing of reactive polymers was demonstrated by analysing the kinetics of cure of a multifunctional epoxy/amine formulation composed of diglycidylether of bisphenol-F (DGEBF) and 4,4′-methylene dianiline (MDA).
A smart grid performs measurement, monitoring and processing of waveforms based on acquisition, analysis, detection and classification techniques [9].
To probe for alteration in DRM containing intralumenal vesicle dynamics, we investigated whether U18666A treatment would affect the release of LF into the cytoplasm by monitoring the processing of one of its targets, the MAP kinase kinase MEK1.
Inhibitory kinetics were determined for all molecules at the optimal pH of GALC, pH 4.6, using enzyme activity assays monitoring the processing of chromogenic substrate 4-nitrophenyl-β d-galactopyranoside (4NβDG) as described previously.
Using an in-gel staining approach for rapid and simple detection of Spinach- or Broccoli-tagged RNAs in cells, we monitored the processing of RNAs tagged with scaffolded aptamers, revealing endonucleolytic cleavage, RNA instability, and poor expression.
