Sentence examples for monitoring phosphorylation of from inspiring English sources

Confluent BAEC were stimulated with 5 mM 2-DG for 5 to 120 min, and AMPK activation was measured by monitoring phosphorylation of AMPKα-Thr172.

JNK activation was determined by monitoring phosphorylation of JNK (Thr183 and Tyr185) and c-Jun (Ser63), which is a substrate of JNK.

JNK activation was determined by monitoring phosphorylation of JNK1 and total expression of this protein.

These observations were confirmed at protein level by monitoring phosphorylation of Stat3 and extracellular signal-related kinase (Erk) 1/2.

Arginase activity in cells lysates was measured by colorimetric determination of urea formed from L-arginine as described previously [ 31]. mTORC1 and S6K1 activity were analyzed by monitoring phosphorylation of S6K1 at threonine-389 (S6K1-T389) and its substrate S6 at serine-235/serine-236 (S235/S236), respectively, by Western blotting.

We conclude that monitoring phosphorylation of paxillin in this pair of cell lines is one approach to assess the bioactivity of LPA.

To investigate this, we monitored phosphorylation of the Nodal intracellular signal transducer Smad2 in oep mutants with a normal or down regulated expression of rasl11b.

Using both untransfected and transfected cells, we monitored phosphorylation of STAT1 and STAT2 under the same experimental conditions as in Figure 3B.

To determine if low pH enhances F− -mediated stress, we treated LS8 cells with F− at pH 6.6 or pH 7.4 and monitored phosphorylation of JNK and c-jun.

That inhibition was not observed strongly suggests that Met oxidation is not affecting our ability to monitor phosphorylation of Ser.

We also monitored phosphorylation of the transcriptional repressor Mig1p, which is targeted by Snf1p under low glucose conditions.