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The changes in the refractive index are proportional to the change in the absorbed mass, thus the analysis allows the monitoring of the interaction process in real-time and the determination of the binding parameters.
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As a superior technique for the label-free monitoring of the cell surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited.
This work has implications for the manufacturing of biologic scaffolds from any tissue or organ, as well as for prediction and monitoring of the scaffold host interaction in vivo.
Unlike other reported nucleic acid hybridization-based nanopore sensors, which rely on time-consuming incubation of the cDNA probe and the target DNA/RNA molecules to achieve sensitive detection, in our nanopore sensor design, detection of a target single-stranded aLF gene segment was achieved by real-time monitoring of the hybridization interaction between the target DNA and the cDNA probe.
Thus, our data suggest that the monitoring of the Dnmt1/PCNA interactions is not a method adapted to analyze the degree of methylation of a specific gene but is adapted to monitor the degree of DNA methylation.
Our data also indicate that the monitoring of the Dnmt1/PCNA interactions was not representative of the increase of the methylation degree of the caspase-8 gene following the Dnmt3a overexpression, but that at middle term (4-days) the number of Dnmt1/PCNA interactions reflected the Dnmt3a-induced increase of 5 mC.
Optical waveguide lightmode spectroscopy (OWLS) is a highly sensitive technique that is capable of real-time monitoring of these interactions.
RSM provides a large amount of information and is more economical approach because a small number of experiments are performed for monitoring the interaction of the independent variables on the response.
Due to the dependence of FRET on proximity, it provides an excellent assay for monitoring the interaction of two proteins in living and intact cells.
Fluorescence-based activity sensors present a novel technology for monitoring the interaction of the transporters with their substrates, the activity of transporters and their regulation in vivo, which is particularly valuable in the context of analytes for which no radiotracers exist, as well as for cell-specific and subcellular transport processes that are otherwise difficult to track.
A large variety of data-driven behavioral models have been developed based on field monitoring of the human-building-system interaction.
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