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Noninvasive monitoring of stem cells, using high-resolution molecular imaging, will be instrumental to improve clinical neural transplantation strategies.
These studies may be greatly facilitated by novel strategies that enable monitoring of stem cell specification in real time.
Many of these strategies have been validated in a wide range of studies evaluating treatment feasibility or efficacy, as well as establishing methods for real-time monitoring of stem cell migration in vivo for optimal therapy surveillance and accelerated development.
These recent studies show the potential of RMS for non-invasive monitoring of stem cell differentiation, which may enable a more efficient optimization of the relevant bioprocesses.
But they are "potentially deleterious," says co-author Anirban Maitra, a pathologist at Johns Hopkins, and underline the need for close monitoring of stem cell cultures as they age.
In vivo monitoring of stem cells after transplantation is essential for a better understanding of their migration/integration into the host tissue.
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Many of these strategies have been validated in a wide range of studies assessing treatment feasibility or efficacy and establishing methods for real-time monitoring of stem-cell migration and fate in vivo.
Medical imaging also plays an important role in individualised drug delivery, by providing information about the locoregional physiological conditions ('regional proteomics' [7]) important for drug targeting [10], by triggering nanotechnological carriers to release active drug [10] or by monitoring differentiation of stem cell based therapeutics [9].
Precisely engineering stem cell fate decisions, however, has remained a challenge due to the lack of analytical tools that allow dynamic monitoring of heterogeneous stem cell populations in situ in real time at the single-cell level.
Noninvasive imaging methods using MRI have the advantage of longitudinal monitoring of transplanted stem cells in animals.
The US/PA imaging was reported as potential tool for in vivo monitoring of mesenchymal stem cells (MSCs) and neovascularization promoted by MSCs [ 15].
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