My own scientific research on aging and longevity -- with an 8-decade monitoring of pathways to long life -- confirms that staying productive is a key element of long-term health and happiness.
For the monitoring of this pathway, the sampling procedure seems to be crucial.
A 10 min pulse of asymmetrically labeled C-1-2-glucose allowed simultaneous monitoring of the pathway flux, origin of labeled metabolites, and thus flux-directionality.
The sensitivity of Raman spectra to metabolic characteristics of microorganisms as well as mammalian cells allows the monitoring of metabolic pathways such as the expression of recombinant proteins.
27 In contrast with Western blotting, the recent progress in flow cytometry allows a quantitative assessment of basal expression of phosphoproteins 70 and treatmentassociated monitoring of signaling pathways.
A Clinical Pathway Steering Committee was established with the following objectives: 1) to follow the methodology, development, implementation and monitoring of care pathways, and 2) to provide a contact for hospital care.
By allowing non-invasive monitoring of biochemical pathways in intact cells and tissues and their modulations under particular pathological conditions, NMR spectroscopy offers novel approaches to detect metabolic alterations associated with malignant phenotypes of ovarian cancer cells in vitro, as a basis for a possible in vivo monitoring of clinical lesions.
β-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation.
Adding the early markers of mitochondria-dependent commitment to apoptosis reported here, to the existing portfolio of apoptosis markers, will provide a non-invasive means for high-resolution, real-time monitoring of the apoptotic pathway.
Here, we describe a FRET-based multi-parameter imaging platform (FMIP) that allows simultaneous high-throughput monitoring of multiple signaling pathways.
