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Höfer, K., Langejürgen, L. V. & Jäschke, A. Universal aptamer-based real-time monitoring of enzymatic RNA synthesis.
The substrates presented here permit continuous fluorescent monitoring of enzymatic sugar combining reactions.
As a consequence, monitoring of enzymatic activities enables a straightforward analysis of the biogas process, avoiding a tedious analysis of numerous chemical parameters.
The monitoring of enzymatic activities using fluorescein-based probes is wide spread.
Isothermal microcalorimetry is a label-free assay that allows monitoring of enzymatic and metabolic activities.
Real-time monitoring of enzymatic conversions and inhibition and formation of complexes is also possible with MS [ 29].
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The second approach is based on the direct monitoring of the enzymatic activity of tyrosinase by detecting amperometrically the reaction products from the enzymatic conversion of l-3,4-dihydroxyphenylalanine (l-DOPA).
Monitoring of the enzymatic reaction in the presence of selected ligands together with structural information obtained from X-ray crystallography analysis have allowed the identification and validation of a novel site of interaction (S2 site).
High throughput, immune-based assays using monoclonal antibodies specific for these immunotoxic peptides would facilitate their detection in food and enable monitoring of their enzymatic detoxification.
This study shows a simple and robust assay for the continuous monitoring of MT enzymatic activity.
Direct monitoring of new enzymatic activities with subcellular resolution is a goal that is yet to be achieved, but holds the promise to improve our understanding of cellular physiology and enzyme function at their correct compartment within cells.
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