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Aβ can be found at low nanomolar or high picomolar concentrations in vivo, but in vitro studies often are performed at micromolar concentrations to enable monitoring of assembly and accelerate the process [ 79].
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While the self-assembly of SbpA can be measured ex situ by scanning transmission electron microscopy (STEM) or atomic force microscopy (AFM), the throughput of these techniques is too low to allow monitoring of self-assembly yield and dynamics over a wide range of conditions.
Real-time monitoring of DNA tile assembly using fluorescence resonance energy transfer revealed that both the number and the relative position of DNA sticky-ends play a significant role in the stability of the final assembly.
Interestingly, a similar distribution was found for upstream sensors and other components of the CWI pathway, further underlining the crucial role of monitoring correct assembly and structural capacity of the newly synthesized cell wall [ 55].
In this paper, the scheme adopted for off-line monitoring of fuel/blanket sub-assemblies, by measuring the coolant flow rate at sub-assembly exit, in a typical sodium cooled fast breeder reactor is presented.
However, such online monitoring of blanket sub-assembly outlet temperature was not envisaged.
The progress and outcome of assembly was monitored by time-resolved X-ray scattering, which allowed us to distinguish between closed shells and incomplete assembly intermediates.
We developed a simple and rapid in vitro assay in which the progress of assembly is monitored by measuring an increase in turbidity, thereby allowing the kinetics of assembly to be determined.
Some checkpoints monitor proper assembly of the mitotic spindle and prevent abnormal segregation of chromosomes during the metaphase to anaphase transition [27].
Piezoelectric quartz crystal impedance (PQCI) technique was employed to monitor the assembly of DNA in real time.
We monitored the assembly of HBV dimer into T = 3 and T = 4 capsids with a nanofluidic device.
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