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Solvent delay was set to 4 min. Selected ion monitoring mode was used for the qualitative and quantitative determination of fatty hydrocarbons.
An ultra performance liquid chromatography-triple quadrupole-linear ion trap mass spectrometry method in multiple-reaction monitoring mode was developed for the rapid determination of 12 bioactive compounds in the leaf and stem of Andrographis paniculata (A. paniculata).
The multiple reaction monitoring mode was used for acquiring data.
The selected reaction monitoring mode was used in this study and therefore made it more specific and sensitive for quantitative analysis (Fig. 3).
GC-MS/MS (Agilent 7000 and Agilent 6890N with Waters Micromass MS) in multiple reaction monitoring mode was used for identification and quantification of 209 PCB congeners as 159 chromatographic peaks.
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Data acquired in multiple reaction monitoring mode are shown in Figure 1A, which shows traces produced by synthetic 2b as well as isolates from control histones (not treated with 3,5-DMAP) and histones from treated cells.
Electrospray ionization at both negative and positive modes and multiple reaction-monitoring mode were applied to detect these analytes.
The multiple reaction monitoring (MRM) mode was selected to monitor the precursor-to-product ion transitions of m/z 329.3 → m/z 229.3 for columbianadin and m/z 217.2 → m/z 202.2 for bergapten (IS) at positive ionization mode.
Multiple-reaction monitoring (MRM) mode was configured to monitor the following mass-to-charge ratio (m/z) transitions: both 404 → 239 (collision energy 25 eV) and 404 → 358 (collision energy 15 eV) for OTA.
Multiple reaction monitoring (MRM) mode was configured to monitor the following mass-to-charge ratio (m/z) transitions: both 404 to 239 and 404 to 358 for OTA, as well as 424 to 250 for U-[C20]-OTA.
Negative electrospray ionization (ESI) in the selected reaction monitoring (SRM) mode was used for MS detection.
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