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The GC-MS is equipped with a RXi-1MS capillary column (20 m × 0.18 mm i.d., film thickness 0.18 μm), with helium as the carrier gas at a flow rate of 0.8 mL min−1, using a selective ion monitoring mode to detect PAHs.
Quantitative analysis was performed in the single ion monitoring mode to maximize sensitivity.
The orbitrap mass spectrometer(26) is a high-resolution instrument, and it was possible in selected ion monitoring mode to unambiguously identify both homozygous and heterozygous sickle cell disease (Δ m 29.9745 Da).
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The single-quadrupole mass spectrometer was operated in the selected ion-monitoring mode to measure positive ions corresponding to [M + H]+ ions of HCQ and the IS at m/z 336.2 and 320.2, respectively.
The mass spectrometer was operated in multiple reaction monitoring (MRM) mode to monitor the precursor ions and the diagnostic product ions of each analyte and IS.
Quantification was performed using multiple reaction monitoring (MRM) mode to monitor transitions of m/z 329.3 → 229.3 for columbianadin and m/z 217.2 → 202.2 for IS at positive ionization mode.
For MS detection, the selected ions monitoring mode seems to be the most efficient.
Carnitine was quantified by positive electrospray ionization in the multiple-reaction monitoring mode relative to an external standard curve (Quest Diagnostics).
All medium and cell lysate samples for TMZ, MTIC, and AIC were analyzed on the same day by high-performance liquid chromatography tandem mass spectrometry (LC/MS/MS) using the multiple reaction monitoring mode specific to each analyte and the internal standard.
Samples were analyzed by UPLC-QqQ-MS in the multiple reaction monitoring (MRM) mode to maximize sensitivity.
All peaks were quantified using multiple reaction monitoring (MRM) mode to study the conversions from parent to product ion (m/z), and data were collected using Analyst version 1.4 (Applied Biosystems).
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