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For internal body temperature monitoring, mice were implanted with Data Science International (DSI) ETA-F20 transmitters.
During continuous monitoring, mice were taken out of the cage and gently handled.
For monitoring, mice were housed in cages over receiver plates connected to a computer where digitized signals were stored.
For continuous food intake monitoring, mice were placed into individual metabolic chambers with free access to water.
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For temperature monitoring experiments, mice were implanted with Mini-Mitters (Starr Life Science Corp., Oakmont, PA) subcutaneously.
For the continuous food intake monitoring study, mice were given 20 mg dustless food pellets (Bio-Serv, Frenchtown, NJ).
Welfare of the animals was monitored, and mice were euthanized as per UK Coordinating Committee on Cancer Research UKCCRR) guidelines (29).
Progression of cancer was monitored, and mice were euthanized when the established criteria for end-stage disease were reached.
The first cohort of animals was closely monitored, and mice were sacrificed upon the occurrence of clinical signs of intracerebral hemorrhages.
To monitor general health, mice were weighed every second day.
To monitor health status, mice were weighed prior to and after testing (Fig. 2).
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