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Multiple reaction monitoring in positive ionization mode was used for the assay.
The detection was performed by multiple reaction monitoring in positive ion mode.
Theoretically, we would consider that prevalences assessed during intensive monitoring in positive flocks be most comparable to active surveillance prevalences.
Analytes were detected using multiple reaction monitoring in positive (TDCPP, D15-TDCPP and D27-TPP) and negative (BDCPP, D10-BDCPP and D10-DPP) ionization modes [see Supplemental Material, Table S1 (http://dx.doi.org/10.1289/ehp.1205316)].
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A: chromatogram of traditional decoction (monitored in negative ion mode); B: chromatogram of dispensing granule decoction (monitored in negative ion mode); C: chromatogram of traditional decoction (monitored in positive ion mode); D: chromatogram of dispensing granule decoction (monitored in positive ion mode).
The samples were monitored in positive mode, resulting in species of 1 mass unit higher than the neutral molecules.
The production of 9-HPOD was monitored at m/z 195 [C9H16O3+Na]+, whereas for 13-HPOD m/z 247 [C13H20O3+Na]+ was monitored in positive mode.
All transitions were monitored in positive ion mode with a dwell time of 10 ms and with Q1 and Q3 set to unit resolution.
The LC runs were monitored in positive ion mode by sequentially recording survey MS scans (m/ z 400 2000), in the ICR cell, while three MS2 were obtained in the ion trap via CID for the most intense ions.
The method was performed under selected reaction monitoring (SRM) in positive ion mode.
The eluted sample was injected into the ion source where the detection of each sphingomyelin species was conducted by multiple reaction monitoring (MRM) in positive mode.
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