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Protection was tested by challenge with mouse-adapted A/FM/1/47 (H1N1) and monitoring for weight loss and survival.
Morbidity associated with CLP surgery was assessed by monitoring for weight loss, abnormal temperatures (normal core temperature range 34 to 37) and other morbidity indicators including ruffled coat, hunched posture, reduced mobility, diarrhoea, abdominal distention, loss of righting ability and laboured respiration; these parameters were scored to calculate a cumulative disease index [25].
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Animals were monitored for weight loss and clinical signs of disease for 40 days to observe both immediate and latent adverse effects.
To study the toxicity following treatment, the animals were monitored for weight loss and/or any decline in their general condition.
Mice were monitored for weight gain as previously described.
The animals were also monitored for weight gain regularly.
Mice were monitored for weight and tumor growth for 30 days.
Following challenge infection, animals were monitored for weight loss and survival.
Animals were monitored for weight loss, temperature and loss of activity weekly during vaccination and daily during challenge.
Mice were monitored for weight loss for 5 days and organs were isolated to measure viral load in naïve and immunized mice.
Mice were next monitored for weight loss and survival after infection with varying doses of the recombinant attenuated virus, vGK5 virus (Fig. 1A).
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