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The prescribing information for quetiapine recommends appropriate clinical monitoring for alterations in lipids, including blood lipid testing at the beginning of and periodically during treatment (Seroquel Prescribing Information 2012).
However, monitoring for alterations in Cry toxin receptors, as in the case of cadherin or ABC transporter-based monitoring methods, would only be effective in detecting resistance to Cry toxins binding to these receptors.
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A representative HeLa cell is shown from such an experiment where two LAMP1 YCaM-positive organeLAMP1 YCaM-positivecatiorganellesonitored for atterations in FRET.
To test whether LPS alone induces comparable CPE-like effects at relevant concentrations in the 3-D intestinal aggregates, we treated the aggregates with increasing amounts of LPS (InvivoGen) and monitored for alterations in morphology indicative of cytotoxicity.
In summary, our data present a novel experimental model for the in vivo monitoring of alterations leading to beta cell failure in obese mice.
Futhermore, this study suggests that the PEA may represent a useful adjunct to the PRA for monitoring alterations in the proportion of drug-resistant virus even when no change in IC50 is apparent.
While the assay described above is ideal for monitoring and quantifying alterations in p-Bsk levels, we note that the technique is applicable to a broad range of cellular events such as the dynamic alterations that accompany cell cycle progression, apoptosis and MAPK signaling.
In particular, it has been used for monitoring collagen alteration in dermal disorders [ 28] or at the tumour-stroma interface [ 33– 35], as well as for monitoring skin aging by measuring the collagen/elastic fibres content [ 36– 36].
Monitoring LCK-pY394 by immunoblot did not reveal convincing evidence for alterations in LCK activity in 90% THEMIS KD cells (Supplementary Fig S3A).
Monitoring these alterations could be useful as a biomarker for characterizing disease progression or determining the efficacy of novel therapies.
OCT is a non-invasive imaging technology that allows for serial monitoring of retinal alterations over time.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com