Sentence examples for monitoring enzyme activity from inspiring English sources

Examinations with tyrosinase (PPO) were equally performed in sodium phosphate buffer (NaPP, 100 mM, pH 6.5) containing 20 μL of a enzyme stock solution (1 mg lyophilized powder dissolved in 1 mL NaPP, with an activity of ≥1000 U mg-1), with monitoring enzyme activity after addition of 5 mM catechol (final concentration, λ = 450 nm) at 25°C.

Later, Ross and colleagues demonstrated a 16-plex gradient elution moving boundary electrophoresis tool for monitoring enzyme activity and inhibition.

This is possibly because of a paucity of convenient and portable assays for monitoring enzyme activity, and of the difficulty of obtaining many of the protein components and complexes in quantities suitable to detailed biophysical characterization.

The simplicity, cheap cost and miniaturization of the short term assays with CSCB indicate that this substrate could be used in the future for monitoring enzyme activity in commercial preparations, before its use on an industrial scale.

The effects of NaCl and CaCl2 on the thermostability of the recombinant AprX-SK37 was determined by monitoring enzyme activity at moderate (40°C) and high (55°C) inactivation temperatures according to the results from the thermal stability assays.

These adducts were shown to be useful substrates for polyP-degrading enzymes, allowing us to monitor enzyme activity spectrophotometrically in real time; furthermore, we used these substrates to identify a new function for the clinically significant enzyme, Nudt2.

Although acetylene reduction was the most convenient way to monitor enzyme activity, the inactivation proceeded equally well when dinitrogen replaced the acetylene or when there was no added substrate beyond the protons in the solution (see Figure S1 of the Supporting Information).

One assay is based on established methods for monitoring ATPase enzyme activity and the second is a novel assay for RecA-DNA filament assembly using fluorescence polarization.

The synthesized compound proved to be a useful substrate for monitoring the enzyme activity of sphingomyelinase by detecting the liberated thiol group with a thiol-sensitive reagent.

The options for implementation of FCM in the development of biotechnological processes detailed in this review are (i) analysing the chemical composition of biomass, (ii) monitoring cellular enzyme activity and cell viability, and (iii) sorting cells to isolate those overproducing the target compound or for the preparation of axenic cultures.

The storage stability of the α-amylase in the toothpaste at room temperature was determined by monitoring the enzyme activity for ten months.