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Guschin, D.Y. et al. A rapid and general assay for monitoring endogenous gene modification.
Guschin, D. Y. et al. A rapid and general assay for monitoring endogenous gene modification.
Of these techniques, gene cassette knock-in is especially useful for monitoring endogenous gene expression and localization, which is essential for functional annotation of endogenous genes.
Toward this goal, we have rationally designed and synthesized a novel two-photon fluorescence probe FC-βgal for monitoring endogenous β-gal in lysosome.
Notably, DFB1 manifests significantly dual-channel and turn-on NIR fluorescent signals simultaneously in response to Cys concentration, which make it favorable for monitoring endogenous Cys activity in vivo.
Recent discoveries of different modes of exocytosis and a plethora of molecules involved in neurotransmitter release has resulted in demand for more rapid and efficient methods for monitoring endogenous glutamate release from various tissue sources.
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The probe CoNO2-HY could monitor endogenous NTR in living HeLa cells.
Importantly, the probe TPE-HY is successfully applied to monitor endogenous NTR in living HeLa cells.
The development and use of selective fluorescent probes offers a technique to monitor endogenous small molecules.
The highly sensitive and selective, cyanine-based Zn2+ fluorescent sensor CTMPA was designed, prepared and utilized to monitor endogenous zinc ions in live cells and organisms.
Recent biochemical analyses also suggested roles for mixed or branched ubiquitin chains, yet without a method to monitor endogenous conjugates, the physiological significance of heterotypic polymers remained poorly understood.
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