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To quantify numbers of cells with epithelial and mesenchymal phenotypes in cultured cells, we employed flow cytometry and started by monitoring cells for the epithelial marker EpCAM as well as monitoring for vimentin or CD44 marking cells with mesenchymal attributes.
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Telekinesis will be commonplace, with appliances controlled by brain scanners; microscopic sensors will continuously monitor cells for signs of danger, extending human life span; internet-enabled contact lenses will tag anything and anyone in sight, enabling omniscience on demand.
We monitored cells for both shape and spreading after incubating on collagen type IV substrates.
In order to determine if the number of cells with DAPI-dense blocks was accumulating over time, we monitored cells for the percentage of DAPI-dense positive nuclei every 10 passage doublings for a total of 70 doublings.
Fears she had spoken under duress were confirmed a year later, when she admitted she'd given her "candid confession" after being held in a heavily monitored cell for a year – "No talking.
MSC are efficient vectors of mycoplasma infection, emphasizing the importance of monitoring cell cultures for contamination.
We further analysed the response of ME180 cells to EGF during infection with meningococci expressing constitutively PilC1 (Nm604a) or PilC2 (Nm910f), by monitoring cell motility for 30 minutes just prior to, or just following, addition of EGF to the cells.
Further, the portable impedance measurement device is a useful analytical tool for monitoring cell growth.
Currently, there is no validated analytical method for monitoring cell or virus growth.
This assay is a modified method for monitoring cell movement that uses different-colored MCF7 cells.
PI (5 ng/ml) was added to the medium for monitoring cell membrane integrity.
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