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Here we developed a method for monitoring cell viability and proliferation in bio-engineered organ constructs using a resazurin perfusion assay.
Antioxidant properties against cytotoxic effect of H2O2 were evaluated by monitoring cell viability.
Chen et al. [46] reported the introduction of multiwall carbon nanotubes modified glassy carbon electrode (GCE) for monitoring cell viability and the effects of antitumor drugs on tumor cells (Leukemia K562 cells) depending on the changes of electrochemical responses.
GloMax Discover is an integrated multimode detection plate reader system that offers scientists an integrated system to easily study cell signaling and metabolism, while monitoring cell viability, apoptosis, and cytotoxicity, or to study gene expression with a wide variety of reporter assays.
During the period of monitoring cell viability, we observed that HFF-MBPsi-4 was growing larger in size.
These results indicate that during the cytolysis process, luciferase does not readily release to the outside and thus the supernatant is not the ideal source for monitoring cell viability based on luciferase assay.
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A constitutive Renilla luciferase vector was included in all mixes (40 1) to normalize transfection efficiency and monitor cell viability.
Resazurin-based redox metabolic assays have been used to monitor cell viability in both two- and three-dimensional cell cultures.
As LPS is a known inducer of TNFα in macrophages, we treated iPS-MΦ with 5 µg/mL TNFα neutralizing antibody and stimulated them with 10 ng/mL LPS and monitored cell viability by video microscopy over 12 h (Supplementary Figure 6C).
The bioelectrochemical assay was optimized to obtain high sensitivity and the method was applied to monitor cell viability and screen susceptibility of metabolically active cells (E. coli, B. subtilis, Enterococcus, P. aeruginosa and Salmonella typhi) to antibiotics such as ampicillin and kanamycin.
In these reports, they monitored cell viability and anticancer drug activities of the cell suspension and immobilized cells; however, they suggested that these electrochemical responses might be related to the oxidation of guanine [45 48].
More suggestions(15)
monitoring cell trafficking
monitoring cell death
monitoring cell response
monitoring cell culture
monitoring cell cycle
monitoring cell metabolization
monitoring cell growth
monitoring cell movement
monitoring cell proliferation
monitoring cell respiration
monitoring cell development
monitoring cell damage
monitoring cell population
monitoring cell division
monitoring cell toxicity
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