To examine the inhibitory efficacy of citreoviridin against cell proliferation, we utilized a real-time cell analyzer to monitor cell responses to the drug over time.
To monitor cell shape in response to this treatment, bright-field images were acquired every second for 2 min using a Axiovert S100 microscope (Zeiss, Oberkochen, Germany) at 40× magnification.
Then we monitored cell cycle recovery in response to MMC in both U2OS and HeLa cells.
52 54 As a result, F-FDG PET may best be used in conjunction with bone scans and to specifically monitor cancer cell response to treatment.
Reliable procedures for detecting and monitoring T cell responses induced by the treatment throughout a clinical trial are needed in order to design rational protocols with increased efficiency.
To identify immune-dominant HBV-specific CTL epitopes, especially epitopes from HBc protein, is therefore necessary for monitoring T cell responses during disease progression, as well as for developing epitope-based therapeutic vaccines against CHB (Inchauspe and Michel, 2007; Gordon et al., 2013; Liu et al., 2013a, b).
Osada and colleagues were the first to monitor NK cell responses in tumor patients vaccinated with DC.
Two synthetic peptides, PEP 544; LMSVYVVELLRSFFYVTE (hTERT (548 566)) and PEP 508; LLDILDTAGHEEYSAMRDQ (KRAS (52 70) Q61H), were used as negative controls in the in vitro T-cell proliferation assays used to monitor T-cell response in blood samples from the vaccinated patients.
By monitoring T-cell responses by flow cytometry, we observed a recall response after recent vaccination against smallpox.
Confluency determination was then applied to the monitoring of cell responses in various relevant scenarios, such as proliferation, growth arrest, cell death and transient morphological changes.
