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With this review, we search to summarize the potential of currently available methods for monitoring cell population heterogeneity as well as model frameworks suitable for describing dynamic heterogeneous cell populations.
The capability of this system is very important when targeting different biological processes and monitoring cell population variations over time.
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Cell growth kinetics were determined by laser scanning florescence microscopy for GFP and by monitoring cell populations.
Quorum sensing (QS) is a mechanism of chemical communication that bacteria use to monitor cell-population density and coordinate group behaviors.
Quorum sensing is crucial for bacteria to monitor cell-population density changes and for synchronizing population-wide gene expression.
GEDI displays a global picture of the state vector relative to both transcriptome and miRNome monitoring the cell population while it moves towards its differentiation fate.
Here we report a different approach enabling correlation of surface receptor engagement and the induced T-cell response through calcium rise monitoring on cell populations brought into contact, in suspension, with model grafted microspheres —the intracellular Ca2+ increase is taken as a reliable indicator of cell activation [18], [19].
This approach enables in vivo monitoring of a cell population deep within the mouse gut that cannot be tracked by light microscopy.
Finally, by monitoring the total cell population in a flow cytometry-based assay, our data show that cells expressing p53-activating peptides rapidly become outgrown by the untransduced cells in the culture.
However, the image quality should still be sufficiently good to monitor whole cell populations, on the one hand and to measure intracellular events on the other.
In this study, we designed the first TCRβ-based oligonucleotide microarray and investigated its specificity, clonality discrimination, sensitivity of detection, and feasibility for monitoring T-cell population diversity in HSCT.
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