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This assay is a modified method for monitoring cell movement that uses different-colored MCF7 cells.
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Picture&+s were taken just after the scratch was made and at 24, 48 and 72 h afterwards, to monitor cell movements.
The second assay is a 'black ink assay' in which the cells were sparsely seeded on an ink-coated surface and then monitored for cell movement, which appears as ink particle cleared tracks on the plastic.
To monitor gastrulation cell movements directly, we have developed time-lapse fluorescent microscopy of zebrafish embryos injected with fluorescent Histone H1 protein, which labels all nuclei (van Eekelen et al., 2010).
Dictyostelium wild-type (A×2) cells, were induced to chemotax by repeated pulsing with cAMP over a 5 h period, and random cell movement was monitored by time-lapse photography, capturing one image every 15 s for 5 min. Cell behaviour was quantified by computer-generated outlines that are used to calculate average cell shape (circularity), protrusion formation and motility.
Cell movement during that period was monitored by an Olympus IX81 motorised inverted microscope taking pictures every 10 min (Liu et al, 2007b).
The 12 biomarkers identified in this study (Table 4) encompass functions of cell movement, inflammation, and immune monitoring that may contribute to cancer development.
Cell Movement.
Stem Cell Movement Faces Setbacks in Mo.
The cell surface undergoes continuous change during cell movement.
Cell movement.
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