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Interestingly, hepatocytes lacking ILK undergo matrix-induced differentiation but their differentiation is incomplete, as judged by monitoring cell morphology and production of albumin.
To determine whether overexpression of human GLTP can alter cell phenotype, GLTP under the control of cytomegalovirus (CMV) promoter was overexpressed in HeLa cells while monitoring cell morphology (Fig. 1).
Cell scattering was tested by monitoring cell morphology after HGF stimulation.
We followed the progress of the differentiation process by monitoring cell morphology, as well as protein and transcript levels of ESC- and NPC-specific markers.
Monitoring cell morphology as well as measuring the number of apoptotic cells by a modified Nicoletti assay 48h after siRNA transfection showed an increase in the number of round and dead cells that was already twofold higher than in control cells, which were transfected with control RNA.
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We periodically monitored cell morphology and observed no changes in the cell lines.
We tested conditioned media on B16 cells as well as C2C12 myoblasts and 10T1/2 fibroblasts and monitored cell morphology, number, and cell cycle status using propidium iodide uptake followed by FACs analysis.
In addition, TPEF and SHG images of hMSCs undergoing differentiation can be used to monitor cell morphology (TPEF) and the deposition of fibrous collagens (SHG) without the need for invasive processing or significant disruption of the culture conditions.
Cells were analysed either by phase contrast microscopy to monitor cell morphology or by fluorescence microscopy to determine mitochondrial morphology.
In this study, we performed the rich to minimal nutrient shift and monitored cell morphology during a 4-day period during which cells underwent changes in morphology along a previously observed timeline.
Measurements on live cells are then presented, which show the possibility of dynamically monitoring the cell morphology by phase during the Raman measurements.
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