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Initial studies monitoring cell binding and uptake clearly showed that the BLM disaccharide containing its carbamoyl moiety was essential for the selective binding and uptake of BLM in tumor cells.
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The FBI assay monitors the inhibition of live S. aureus cells binding to fibrinogen as shown schematically in Figure 4.
The recent advent of peptide MHC tetramers has provided a new and effective tool for studying antigen-specific T cell populations through monitoring tetramer binding to T cells by flow cytometry.
Monitor cell interactions.
DOI: http://dx.doi.org/10.7554/eLife.09384.012 To define the Gravin-Plk1 interface during mitosis, we monitored cells depleted of Gravin and rescued with kinase-binding mutants of the anchoring protein.
To determine how long C-ATR takes to reach saturation binding to the pituitary cells, we monitored the binding of 1.0 ppm C-ATR to pituitary cells over a 3-hr time course.
Uptake of H-dopamine (10 nM final concentration, 48 Ci/mmol, Perkin Elmer) into suspended cells expressing human DAT was measured for 5 min at 21°C, and to monitor cocaine analogue binding, cells were incubated with 4 nM H-CFT (2β-carbomethoxy-3β-[4-fluorophenyl]-tropane, 85.9 Ci/mmol, Perkin Elmer) for 20 min at 21°C; for saturation analysis, 0.1 100 nM non-radioactive CFT was also present.
To this end, we have developed a new approach that now enables monitoring both binding to and activation of T cells by peptide MHC tetramers at the single-cell level.
Using immortalized Jurkat T cells, we monitored both binding and activation events, as seen by changes in the intracellular calcium concentration.
It can also be used to investigate the effectiveness of early-phase treatment response by monitoring the binding of drug molecules to the tumor cells.
Interestingly, fluorescent ligands have been developed for a variety of GPCRs in order to monitor ligand receptor binding in living cells.
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