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For each of these testing periods, vital signs were assessed using the ICU monitoring at rest, during a nociceptice procedure and 20 min postprocedure.
Each subject underwent two hours of electrocardiogram monitoring at rest.
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Pulse oximetry, ECG, and BP were monitored at rest, during exercise, and at recovery.
Cardiac function was monitored at rest and during exercise using a 12-lead electrocardiogram.
Heart rate, and blood pressure (sphygmomanometer and a stethoscope) will be monitored at rest and during exercise.
In addition, in this preliminary study, patients were monitored at rest and thus, we can make no assumptions concerning performance with patients moving around in a truly ambulatory context.
Blood pressure, electrocardiography (GE healthcare, Cardio Soft EKG, Freiburg, Germany) and pulse oximetry were monitored at rest, continuously during the test and for 3 min into the recovery phase.
We seek to minimize using the recommended approaches, i.e., monitoring the mice at rest at a defined time during the day for a short recording period.
The monitored vessel diameters at rest ranged between 40 and 150 μm.
Since incomplete evaluation of pain and its consequences, were considered as flaws in previous studies, we carefully monitored pain, not only at rest but also on mobilization.
To acquire information of baseline and to monitor membrane potential (Vm) at rest, cells were recorded for periods of 20 min using the current-clamp mode of the whole-cell configuration of the patch-clamp technique.
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