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Participants enrolled in the REACH cohort were invited to participate in a substudy focused on intensive adherence monitoring, as previously described [25], [28], [29].
Dried samples were dissolved in 1% (v/v) n-heptafluorobutyric acid and analyzed by reverse-phase HPLC detecting hydroxylysyl pyridinoline and lysyl pyridinoline by fluorescence monitoring as previously described [21].
All patients received general anesthesia with standard hemodynamic and temperature monitoring, as previously described (5).
Cardiac function was determined with echocardiography and invasive hemodynamic monitoring, as previously described (Yu et al, 2013).
Sheep (weighing 30 to 40 kg) were surgically prepared and chronically instrumented for haemodynamic monitoring as previously described [ 13, 14].
We can therefore assume that drift is not related to the duration of monitoring, as previously indicated [ 7].
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However, the majority of these models only make use of measured data from automatic monitors, which as previously discussed are spatially sparse.
ATPS were prepared and monitored as previously described.
ATPS for this step were prepared and monitored as previously described.
Mice were monitored as previously described.
Conidiophore was monitored as previously described [32].
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