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Solute transport was quantified by monitoring accumulation of fluorescence in a saline bath circulated around the explant.
Reactivation of the transgene is easily scored by visually monitoring accumulation of anthocyanin pigmentation.
In our previous experiments, we found that supplementing a high-cholesterol diet (HCD) with red fluorescent CE enabled monitoring accumulation of vascular lipid deposits in live transparent zebrafish larvae (Fang et al., 2011; Stoletov et al., 2009).
Since there is no convective transfer of fluid between the probe and the environment, this type of probe is particularly useful when measuring in small confined volumes or in vivo, for example monitoring accumulation of chemotherapeutics in breast tissue [ 17, 18].
Three cytological markers of PTI/HR induction were initially analyzed, by monitoring accumulation of ROS with 3,3 diaminobenzidine (DAB) [ 28], deposition of callose at plant cell wall with aniline blue [ 29, 30], and host cell death with lactophenol trypan blue [ 31, 32].
We investigated progression through the meiotic program by monitoring accumulation of messages from several meiosis-specific genes: the DSC targets psm3+, rec12+ and dfp1+, a non-DSC early gene rec25+, middle meiotic genes mei4+, and mei4+-dependent transcripts cdc25+ and mde10+ (Fig. 3).
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The top was then imaged using a fluorescence microscope to monitor accumulation of particles.
We recently used γH2AvD (Drosophila γH2AX) signals, a marker of DSBs, to monitor accumulation of DNA damage in midgut ISCs in relation to age and oxidative stress [ 24].
Immediately after the addition of lamotrigine to the cultures, cells were pulse labeled with [C]-uridine to monitor accumulation of non-native ribosomal particles.
An alternative approach to large studies in solving uncertainty is to use CMA to monitor accumulation of treatment effects in clinical trials [ 38].
The relative expression of the genes from each sample set was averaged and statistical analysis for significance was performed using a Student's t-test.> The AdipoRed assay was employed to monitor accumulation of intracellular lipid at 7 and 21 days of adipogenic cell culture media consistent with a protocol directing differentiation into the adipocyte phenotype.
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