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The triad is highly photo-stable as has been shown by monitoring absorption spectra under prolonged blue-light exposure.
Lactate levels were determined using a standard curve by monitoring absorption at 340 nM and the rate of lactate production was calculated as a function of time and cell concentration [37].
Optical sensing platforms employ various methods, including refractive index change monitoring, absorption, and spectroscopic-based measurements.
Fractions containing DNA were identified by monitoring absorption at 260 nm and then combined and concentrated under reduced pressure for subsequent nanopore assembly.
Finally, we show that this fiber-optic system is capable of monitoring absorption changes of about 3 × 10−3 at every 10 ms, which is of great benefit for the development of fast PALM.
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Chromatography was performed on a SMART analytical Superdex G75 column on a SMART system from Amersham Pharmacia (30 cm length, bed volume 2.4 ml) using a flow rate of 100 µl/min and with monitoring of absorption of elution carried out at 257 nm (corresponding to the absorption of phenylalanine).
Therefore, the extent to which DNA is single-stranded or double-stranded can be determined by monitoring UV absorption.
Eluted products were detected by monitoring UV absorption at 205 nm.
Eluted carbohydrates were detected by monitoring UV absorption at 205 nm.
The amount of product formed was followed by monitoring the absorption changes at 256 nm.
By monitoring the absorption alterations at 471 nm we assess the activity of chlorophylls, as shown in Table 4.
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