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Cells exhibiting a green fluorescence signal, revealing of caspase-3 activation, were monitored via flow cytometry (Cytomics FC500, Beckman Coulter).

The mode of cell death induction, apoptosis or necrosis, was monitored via flow cytometric assay after co-staining of cells with Annexin V-FITC and PI.

Here, the DNA content was monitored via flow cytometry in addition to forward scattering (FSC) giving relative information about cell size (Müller and Nebe-Von-Caron [2010]) (Figure 3).

Additionally, therapeutic effects may be monitored via flow cytometric analysis of GPI deficiency.

(C ) Uptake of fluorescently labelled dimeric HpHb into live cells was monitored via flow cytometry across a range of 1 62.5 nM.

GFP expression of transfected CHO and HEK 293S cells was monitored via flow cytometry on a CyAn ADP Analyzer (Beckman Coulter, Krefeld, Germany).

Similar(54)

Anaerobic enzyme solutions were monitored via stopped flow methods following rapid mixing with anaerobic buffer that contained 240 μM NADPH for a final NADPH concentration 120 μM in 200 mM buffer of the appropriate pH.

The formation of the C4a-OOH(D) intermediate and its subsequent conversion to FAD were monitored via stopped-flow using air-saturated buffer ±15 mM l-Lys or l-Orn at near neutral pH/pD (6.8).

In the CMV group, suppression of diaphragm muscle activity was monitored via airway pressure and flow waveform as previously described [ 1] and reconfirmed in sonomicrometry experiments in which diaphragm electrical activity was monitored.

Coronary flow was monitored via volumetric analysis of coronary effluent.

In this assay, the femoral artery was surgically severed in both Nrp1 VEGF− and control mice, and blood flow recovery was monitored via deep penetrating laser Doppler imaging.

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