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ECG was monitored using limb leads.
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Blood flow in the hind limb was monitored using laser Doppler flowmetry (moorLDI2 imager) and was also confirmed by the change in the leg skin color.
Pulse oximetry (CANL-425 V, Med Associates, St-Alban, VT, USA) was monitored using a probe taped on the hind limb to assure proper blood oxygenation (95 99%).
Arthritis severity was monitored using a conventional arthritic score (1 point for each limb with at least one affected toe, 2 points if the whole paw is swollen) and a modified score (1 point if one toe per limb is affected, 2 points if more than one toe per limb is affected or a moderate paw swelling is observed, 3 points if the entire paw is swollen, 4 points if the paw is severely swollen).
The systolic and diastolic blood pressures and the pulse rate were monitored using a noninvasive monitor, and the cuff/cables were applied to the unaffected upper limb.
Heart electrical activity and respiration were monitored, using an in-house electrocardiography and respiratory gating device, via subcutaneous needle electrodes inserted into the fore limbs, and a motion loop on the abdomen, respectively.
Lentiviral transduction was monitored using anti-mCD24.
This is because the cat constantly monitors its flight and uses limbs and tail to steer, making sure it lands on its feet.
Six muscles in the right upper limb were monitored bilaterally using surface electromyography.
Anaesthesia depth was monitored by limb withdrawal using toe pinching.
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