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As a metric of success in achieving reliable, consistent and efficient incorporation of hESC into a single aggregate per well, aggregate size (cross-sectional area) was monitored using light microscopy under various conditions.
The culture medium was refreshed on day 3, and cell differentiation was monitored using light microscopy.
The S236A construct formed a complex with actin whose ATP-induced dissociation was monitored using light scattering.
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The health and activity of these cells is characterized through a colorimetric metabolic assay that is monitored using light-emitting diodes and photodetectors.
Chlamydia infected McCoy cells were constantly monitored using inverted light microscopy during the time course of 192 h p.i., which revealed formation of multiple small non-fusogenic or single "empty" looking giant inclusions (Fig 11).
During fractionation experiments, the average particle size was monitored using dynamic light scattering (DLS) with a Brookhaven BI-200SM.
The size of the ternary complexes was monitored using dynamic light scattering and the size distribution of the complex's populations was determined by size distribution processor analysis.
After washing with PBS and deionized water, the sections were silver-enhanced using a SEKL 15 silver enhancing kit (BBInternational) according to the manufacturer's instructions, with progress being periodically monitored using a light microscope.
Particle concentration within the breathing zone of the mice was continuously and cumulatively monitored using a light scatter device (DustTrak 8520; TSI, St Paul, MN) and maintained by controlling the airflow into the nebulizer.
Real-time personal particle exposure was continuously monitored using the light-scattering technology of a DustTrak™ Aerosol Monitor (TSI, Inc., St . Paul MN) fitted with an inlet impactor designed to separate particles with a median aerodynamic diameter of less than 2.5 μm (PM2.5).
The cells were regularly monitored using an inverted light microscope, and the culture medium was changed every 2 days.
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